{"id":1421,"date":"2020-08-03T10:24:28","date_gmt":"2020-08-03T10:24:28","guid":{"rendered":"http:\/\/ptbf.pl\/?p=1421"},"modified":"2020-09-30T12:13:13","modified_gmt":"2020-09-30T12:13:13","slug":"poszukujemy-stypendystow-studentow-doktorantow","status":"publish","type":"post","link":"https:\/\/ptbf.pl\/?p=1421","title":{"rendered":"Poszukujemy Doktorant\u00f3w"},"content":{"rendered":"

Poszukujemy Doktorant\u00f3w<\/strong> do wsp\u00f3\u0142pracy w realizacji projektu badawczego NCN OPUS18 pt. \u201eRola mitochondrialnych kana\u0142\u00f3w potasowych w uszkodzeniach wywo\u0142anych py\u0142ami miejskimi (PM) \u2013 poszukiwanie nowej strategii cytoprotekcji\u201d realizowanego w Konsorcjum Szko\u0142a G\u0142\u00f3wna Gospodarstwa Wiejskiego w Warszawie i Instytut Biologii Do\u015bwiadczalnej im. M. Nenckiego w Warszawie.<\/p>\n

Studia b\u0119d\u0105 realizowane w Szko\u0142ach Doktorskich:<\/strong><\/p>\n

Warszawska Szko\u0142a Doktorska Nauk \u015acis\u0142ych i BioMedycznych<\/a>
\nSzko\u0142a Doktorska SGGW<\/a><\/strong><\/p>\n

Szczeg\u00f3\u0142owe informacje: piotr_bednarczyk@sggw.edu.pl, tel.: 22 59 386 20.<\/p>\n

Doktorat 1: <\/strong>PhD student will be mainly focused on regulation of the mitochondrial potassium channels by particulate matters (PMs, one of the main smog component) and other modulators. Furthermore, regulation of mitochondrial functions by particulate matters will be studied. Additionally, the fellow will analyze modulation of ion transport across epithelial tissues\/cells by PMs and verify the role of potassium channels in this process. It is planned to use mitochondria\/mitoplasts from various cellular models (also newly developed in the project).
\nMain experimental techniques include: mitoplast patch-clamp, ion transport across monolayer, Ussing experiments, high resolution respirometry, mitochondrial membrane potential measurement, mitochondrial ROS synthesis, cell cultures in vitro<\/em>, mitochondria and mitoplast preparation.
\nWe are looking for creative, willing to cooperate team player with biological, chemical or biophysical background. We expect basic knowledge in biochemical and biophysical techniques. Experience in electrophysiology will be additional advantage. Good written and verbal communication skills in English are recommended.<\/p>\n

Doktorat 2: <\/strong>PhD student will be mainly focused on analysis of mechanisms of PMs induced cell death and cytoprotective role of the mitochondrial potassium channels against PMs induced injury. We plan to use existing experimental models based on cell lines of epithelial origin. Additionally, the fellow will work on development of new cellular models using modern tools (e.g. CRISPR\/Cas9 technology, gene silencing, gene overexpression). The student will analyze among others cell survival, expression of selected genes, protein levels (with special focus on mitochondrial fraction), composition of mitochondrial respiratory chain complexes.
\nMain experimental techniques include: molecular biology (e.g. quantitative PCR, mutagenesis), cell cultures in vitro<\/em>, cell death assays, mitochondria preparation, SDS and Blue-Native Page, Western Blot.
\nWe are looking for creative, willing to cooperate team player with biological or biotechnological background. We expect basic knowledge in molecular biology and biochemical techniques. Good written and verbal communication skills in English is recommended.<\/p>\n

Wi\u0119cej szczeg\u00f3\u0142\u00f3w o projekcie tutaj<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"\"\"<\/a>

Poszukujemy Doktorant\u00f3w do wsp\u00f3\u0142pracy w realizacji projektu badawczego NCN OPUS18 pt. \u201eRola mitochondrialnych kana\u0142\u00f3w potasowych w uszkodzeniach wywo\u0142anych py\u0142ami miejskimi (PM) \u2013 poszukiwanie nowej strategii cytoprotekcji\u201d realizowanego w Konsorcjum Szko\u0142a G\u0142\u00f3wna Gospodarstwa Wiejskiego w Warszawie i Instytut Biologii Do\u015bwiadczalnej im. … Czytaj dalej →<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[3],"tags":[],"class_list":["post-1421","post","type-post","status-publish","format-standard","hentry","category-aktualnosci"],"_links":{"self":[{"href":"https:\/\/ptbf.pl\/index.php?rest_route=\/wp\/v2\/posts\/1421","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ptbf.pl\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ptbf.pl\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ptbf.pl\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/ptbf.pl\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1421"}],"version-history":[{"count":7,"href":"https:\/\/ptbf.pl\/index.php?rest_route=\/wp\/v2\/posts\/1421\/revisions"}],"predecessor-version":[{"id":1438,"href":"https:\/\/ptbf.pl\/index.php?rest_route=\/wp\/v2\/posts\/1421\/revisions\/1438"}],"wp:attachment":[{"href":"https:\/\/ptbf.pl\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1421"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ptbf.pl\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1421"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ptbf.pl\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1421"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}