Department of Physics and Biophysics/Institute of Biology
Warsaw University of Life Sciences, Warsaw, Poland
We are seeking a highly motivated and talented PhD student to join our research team at the Department of Physics and Biophysics, Institute of Biology at Warsaw University of Life Sciences. This position is a valuable opportunity for a passionate individual to contribute to cutting-edge research in the field of T cell biology and immune response, with a specific focus on the role of Transient Receptor Potential Ankyrin 1 (TRPA1) and Melastatin 2 (TRPM2) channels in relation to hypoxia and the regulation of immune response.
Project Overview:
The primary objective of this project is to investigate the role of TRPA1 and TRPM2 channels in the physiology of T cells, particularly in relation to hypoxia and their potential involvement in the regulation of immune response. T lymphocytes, as crucial components of the adaptive immune system, play a pivotal role in combating pathogens, including viruses, and are involved in cancer and autoimmune disorders. However, the intricate regulation of T cell functioning is influenced by multiple factors, such as temperature, pH, and hypoxia. Hypoxia, in particular, is known to have a detrimental impact on T cell physiology, affecting their activation, cytotoxicity, and metabolism. The project aims to explore the link between hypoxia-sensing, TRPA1 and TRPM2 channels, and their potential influence on T cells physiology.
Responsibilities:
- Isolation of primary T cells from human blood samples and T cells culture,
- Performing molecular biology techniques, including RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) analysis,
- Conducting Western Blot analysis to determine protein expression levels,
- Performing enzyme-linked immunosorbent assay (ELISA) for cytokine analysis,
- Employing flow cytometry to analyze T cells phenotype and function,
- Designing and executing experimental protocols, including data collection, analysis, and interpretation
- Collaborating with fellow researchers, presenting findings at scientific conferences, and contributing to scientific publications
- Conducting comprehensive literature reviews to establish a strong foundation in the research area
Requirements:
- A Master’s degree (M.Sc) in biology, biotechnology, or a related discipline
- Strong background in cell culture and molecular biology techniques, experience in T cell isolation, cell culture, qPCR, Western Blot, ELISA or flow cytometry techniques will be advantageous
- Excellent analytical and problem-solving skills
- Ability to work independently and as part of a team
- Fluency in written and spoken English
Benefits:
- Gross salary 5000 PLN per month,
- Collaboration opportunities with experts and researchers in the field,
- Possibility for professional development, including workshops and conferences,
- Supportive supervisors and mentors to guide you throughout your PhD journey,
Application Procedure:
To apply for this position, please submit the following documents as a single PDF file via email to joanna_bujak@sggw.edu.pl:
- a detailed curriculum vitae (CV) highlighting your academic qualifications and research experience
- a cover letter describing your research interests, motivation, and why you believe you are a suitable candidate for this position
- copies of academic transcripts and degree certificates
- contact information of two professional references who can provide letters of recommendation
- selected candidate is required to apply for admission to the doctoral school (please find more information about recruitment to the doctoral school here: https://www.sggw.edu.pl/en/home/science/doctoral-school/recruitment-to-doctoral-school/ )
Application Deadline: August 15, 2023
